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Small-scale yeast cell disruptions

Depto. Biologia Celular bc2biced at lucano.uco.es
Tue Aug 29 13:45:55 EST 1995

Jon, to obtain yeast's cell homogenate I use normally glass beads (0.5 mm 
diameter) and vortexing. This method is very quick and provides a good cell 
extract. If you need more information about this method you can reed this 
reference: "Isolation and Biochemical Characterization of Organelles from 
the Yeast Saccharomyces cerevisiae" Erwin Zinser YEAST vol 11 1995 pp 
Bye, good luck

Carlos Santos
Dept Biologia Celular
Facultad de Ciencias
Cordoba SPAIN

On 26 Aug 1995, Jon Stewart wrote:

> We are expressing a monooxygenase protein in S. cerevisiae and would
> like to follow the time course of enzyme expression.  What is the 
> preferred method of disrupting yeast cells on a small scale that also
> preserves enzyme activity?  Thanks for your advice.
> Jon Stewart
> University of Florida

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