You can probably glass bead-lyse them if you keep the cells cold in
between. Chill 3-6 OD's of cells per time point and concentrate them in
about 200 ul of your lysis buffer. Add glass beads to 100 ul or so and
Vortex for 5 30s cycles, alternating with 30s on ice to cool. This is
used on both large and small scales, and is easy enough to try once.