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Small-scale yeast cell disruptions

Kevin Morano kamorano at ucdavis.edu
Mon Aug 28 12:47:14 EST 1995


Jon-

You can probably glass bead-lyse them if you keep the cells cold in
between.  Chill 3-6 OD's of cells per time point and concentrate them in
about 200 ul of your lysis buffer.  Add glass beads to 100 ul or so and
Vortex for 5 30s cycles, alternating with 30s on ice to cool.  This is
used on both large and small scales, and is easy enough to try once.

Kevin Morano



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