We've had success with doing subcellular fractionation by sucrose gradients. A
good reference is Kolling and Hollenberg, EMBO 13: 3261 (1994). I'd also be
happy to lend any advice that I can.
In message <41japj$oou at news.service.uci.edu> Jonathan Claypool writes:
> Hello, What do people think are the best protocols for yeast subcellular
> fractionation? I am
> looking to determine the location of components of a yeast retrotransposon.
> Any help is
> Jonathan Claypool
>eabg153 at rigel.oac.uci.edu