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Direct cell to cell Yeast-E.coli shuttle by electroporation?

Tomas Drgon tomasd at bdg10.niddk.nih.gov
Wed Aug 23 18:59:31 EST 1995


In article <41dfuk$j0s at fremont.ohsu.edu>, Nick Kaplinsky
<kaplinsk at ohsu.edu> wrote:

> You may want to try using a Promega miniprep column (~$1) to purify your 
> yeast plasmid.  The resin specifically binds plasmid DNA and whatever 
> the yeast factor is that inhibits transformation gets washed away.  I 
> even use the included alkaline lysis reagents on my yeast (no need for 
> zymolyase or glass beads/phenol/chloroform) - I get 50-100 colonies by 
> transforming with 5ul of the DNA I get from the prep this way (from a 
> 2ml liquid culture).  I am using this for screening two-hybrid clones, 
> and by plating my E. Coli onto Leu- plates I do my curing in the same 
> step as the tranformation.
> Nick

Hi.
You mean you just run the whole procedure as for E.coli?? Sonds cool.

Tomas

-- 
I speak for nobody but myself and my pet frog.



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