In article <41dfuk$j0s at fremont.ohsu.edu>, Nick Kaplinsky
<kaplinsk at ohsu.edu> wrote:
> You may want to try using a Promega miniprep column (~$1) to purify your
> yeast plasmid. The resin specifically binds plasmid DNA and whatever
> the yeast factor is that inhibits transformation gets washed away. I
> even use the included alkaline lysis reagents on my yeast (no need for
> zymolyase or glass beads/phenol/chloroform) - I get 50-100 colonies by
> transforming with 5ul of the DNA I get from the prep this way (from a
> 2ml liquid culture). I am using this for screening two-hybrid clones,
> and by plating my E. Coli onto Leu- plates I do my curing in the same
> step as the tranformation.
You mean you just run the whole procedure as for E.coli?? Sonds cool.
I speak for nobody but myself and my pet frog.