On 23 Aug 1995, Mark Flory wrote:
> Date: 23 Aug 1995 10:32:16 -0700
> From: Mark Flory <mflory at u.washington.edu>
> To: yeast at net.bio.net> Subject: pombe transformation
>>>> I am trying a high copy suppression screen in pombe and need a
> consistent method for transformation that gives at least reasonably high
> efficiency. I have found that the LiAc/PEG protocol geared for
> S.cerevisiae does not in my hands give high efficiency with pombe. Does
> anyone have any suggestions for methods I might try?
>> Mark Flory, University of Washington
> Dept. of Biochemistry
> (206) 543-5345/mflory at u.washington.edu>>Try the protocol in BioFeedback VOL 15 No.4 (1993) under "Rapid
Transformation of Cryopreserved Competent S.pombe Cells".
It works very well in my hands.
University of Montreal