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pombe transformation

Jannatipour Mehrdad jannatim at ERE.UMontreal.CA
Wed Aug 23 13:41:44 EST 1995


On 23 Aug 1995, Mark Flory wrote:

> Date: 23 Aug 1995 10:32:16 -0700
> From: Mark Flory <mflory at u.washington.edu>
> To: yeast at net.bio.net
> Subject: pombe transformation
> 
> 
> 
> I am trying a high copy suppression screen in pombe and need a 
> consistent method for transformation that gives at least reasonably high 
> efficiency.  I have found that the LiAc/PEG protocol geared for 
> S.cerevisiae does not in my hands give high efficiency with pombe.  Does 
> anyone have any suggestions for methods I might try?
> 
> Mark Flory, University of Washington
>  Dept. of Biochemistry
> (206) 543-5345/mflory at u.washington.edu
> 
> 
Try the protocol in BioFeedback VOL 15 No.4 (1993) under "Rapid 
Transformation of Cryopreserved Competent S.pombe Cells".
It works very well in my hands.
Good Luck
                                                  Mehrdad Jannatipour
                                                  University of Montreal



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