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I am looking for a mutant ecoli...

jeffric at kea.lincoln.ac.nz jeffric at kea.lincoln.ac.nz
Wed Aug 23 19:42:32 EST 1995

	*Wanted*				(cross-posted)

An E.coli that has a high expression of the lac operon 
specifically high expression of the lac-permease and b-galactosidase 
proteins), and hence has unrestricted growth on lactose. However (and 
heres the cruncher) the strain must also have mutations in its pts 
carbohydrate system (specifically HPro and enz1) so it cannot utilise 
pts carbohydrates to any great extent compared to lactose and must 
also have mutations in its galactose permease system so it cannot 
readily use galactose as a carbohydrate source. 

That is does a strain exist that can preferentially use lactose over
other common carbohydrates as a primary energy source? 

Where is this strain? 

(Sorry for the cross-post; a (polite) recommendation about appropriate
groups would be appreciated.)

Please reply by *mail* to jeffric at kea.lincoln.ac.nz


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