I have just started working on Pichia (using the Invitrogen kit) and am
encountering a problem with my GS115 strain growing in clumps of about
100 to 1000 cells. Invitrogen say that this is not normal and suggested that I
try to make a fresh batch of medium (YPD) and shake the culture harder. Ive
done both but the problem persists. Has anyone got any ideas?
Paul Lowings (mbjpl at uk.ac.dl.seqnet)