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pombe transformation

Mark Flory mflory at U.WASHINGTON.EDU
Wed Aug 23 12:32:16 EST 1995

I am trying a high copy suppression screen in pombe and need a 
consistent method for transformation that gives at least reasonably high 
efficiency.  I have found that the LiAc/PEG protocol geared for 
S.cerevisiae does not in my hands give high efficiency with pombe.  Does 
anyone have any suggestions for methods I might try?

Mark Flory, University of Washington
 Dept. of Biochemistry
(206) 543-5345/mflory at u.washington.edu

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