I am trying a high copy suppression screen in pombe and need a
consistent method for transformation that gives at least reasonably high
efficiency. I have found that the LiAc/PEG protocol geared for
S.cerevisiae does not in my hands give high efficiency with pombe. Does
anyone have any suggestions for methods I might try?
Mark Flory, University of Washington
Dept. of Biochemistry
(206) 543-5345/mflory at u.washington.edu