> To: yeast at net.bio.net> From: boraston at unixg.ubc.ca> Subject: Pichia pastoris - protease mutants
> I'm battling a degradation problem with expressing a protein in
> pichia. The battery of techniques used to try to reduce proteolysis
> has failed. Does anyone know of how to get a hold of one/some
> protease deficient mutants and has anyone worked with these before?
when you say:
> Please email me directly: boraston at unixg.ubc.ca. ^^^^^^^^^^^^^^^^^^^^^^^^
are you saying:
1) please e-mail me and I will be happy to summerize to the group,
and this way we will all save bandwith ...
or are you saying:
2) I don't bother reading this group, so when you reply to me, please
e-mail me directly, so I won't have to put myself out ...
| B.F. Francis Ouellette
|||francis at ncbi.nlm.nih.gov