Daniel Harkness (harkness at bio.bu.edu) wrote:
: John R. Thompson (jrt at intac.com) wrote:
: : Has anyone mixed yeast cells and E.coli cells and electroporated to effect
: : a direct cell to cell transfer for shuttling plasmids? I can't recall if
: : seen the concept published or just thought it should be possible.
: : John Thompson
I have never tried the protocol, but there is a reference for this:
Marcil,R. and Higgins,D.R. (1992). Direct transfer of plasmid DNA from
yeast to E.coli by electroporation. Nucleic Acids Res. 20:917
Note: Only 2 micron plasmids were tested.
---------------------Al (aplummer at sprint.uccs.edu)