John R. Thompson (jrt at intac.com) wrote:
: Has anyone mixed yeast cells and E.coli cells and electroporated to effect
: a direct cell to cell transfer for shuttling plasmids? I can't recall if
: seen the concept published or just thought it should be possible.
: John Thompson
In "Guide to Yeast Genetics and Molecular Biology" by Guthrie and Fink
there is a section on conjugation between bacteria and yeast in terms of
transmission of plasmids (page 187). Basically they say mating only occurs
on a solid substrate to ensure yeast/bacteria contact. I didn't read anything
on electroporation but then again the copyright on this edition is '91. If
you do come across a reference for electroporation of yeast and bacteria
please post. I hope this helps.
Dept. of Cell and Molecular Biology
5 Cummington St.
Boston, MA 02215
email: harkness at bio.bu.edu