IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

2 hybrid-integrating

Susan Heimer sheimer at umabnet.ab.umd.edu
Mon Aug 7 14:17:59 EST 1995

Greetings 2-hybrid system users:

	I am using the Brent 2-hybrid system to demonstrate an 
interaction between 1 subunit of a trimer and an unrelated protein.  
However, it is not clear whether this subunit will fold properly in the 
absence of the other 2 subunits of the trimer.  It was suggested to me to 
integrate the other 2 subunit genes if I find this is the case.

	Is anyone aware of appropriate constructs, strains (or 
literature) that are compatible with integration in the Brent 2-hybrid 

	Any feelings about using pSH18-34spe (Steve Hanes) to integrate 
both my subunits and the lacZ reporter? 

	These are prokaryotic genes.  Do I require a yeast promoter for 

Thank you for any help you can offer!!!!!


Susan Heimer
University of Maryland at Baltimore

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net