Greetings 2-hybrid system users:
I am using the Brent 2-hybrid system to demonstrate an
interaction between 1 subunit of a trimer and an unrelated protein.
However, it is not clear whether this subunit will fold properly in the
absence of the other 2 subunits of the trimer. It was suggested to me to
integrate the other 2 subunit genes if I find this is the case.
Is anyone aware of appropriate constructs, strains (or
literature) that are compatible with integration in the Brent 2-hybrid
Any feelings about using pSH18-34spe (Steve Hanes) to integrate
both my subunits and the lacZ reporter?
These are prokaryotic genes. Do I require a yeast promoter for
Thank you for any help you can offer!!!!!
University of Maryland at Baltimore