I am interested in getting a two hybrid vector that allows a
C-terminal fusion of either the Activation domain or the Gal4 DNA
binding domain for use in the Elledge two hybrid system. I think that
the interaction that I am looking for may involve the very N-terminus of
the target protein so I would like to avoid an N-terminal fusion. Since
I am testing a specific set of proteins for interaction (as opposed to
screening a library) I can use either type of fusion. Thanks.