Colin MacDiarmid wrote:
>allele, but found recently that this is associated with a petite phenotype (sin>ce the his3 deletion removes some of the promotor of the adjacent pet56 gene) ->so if I use a diploid his3D200 strain I cant sporulate it!...
I don't know about everyone else, but n my hands the his3D200 allele is not as-
sociated with a petite phenotype. Also, I have sporulated several his3D200/
his3D200 diploids of various genetic backgrounds, without a problem. So your
sporulation problem has nothing to do with the his3D200 allele. I would get another strain. Our lab has several his3D200 strains, and I can send some if you
Frederick Cancer Research and Development Center