Dear Fizzies,
I have used 5-FOA selection extensively on S. pombe and it works
well. I use it to select for recombinants between plasmids and a ura4+
marked deletion in the genome as well as for 2-step gene replacements. I
use YEA plates containing 500 mcg/ml 5-FOA. Make the plates by
autoclaving 2X YE media, cool, then add an equal volume of filter
sterilized 1 mg/ml 5-FOA. Patch out your Ura+ strain on 5-FOA pretty
densely, when colonies come up re-streak on 5-FOA, finally test on EMM
-ura. Good luck!
Sincerely,
Mike Moser Tel: 206-543-5354
UW Department of Biochemistry FAX: 206-685-1792
Box 357350 moser at u.washington.edu
Seattle, WA 98195-7350 Make peace my beast is yeast
