Hi, all the pombe ppl out there, I need your help.
I have worked mostly with cerevisiae so far. Now time has come for me to
do some experiments with pombe. The problem I'm having is with the
transformation of a ts pombe strain. I have tried a couple of times using
the yeast transformation kit from ZYMO RESEARCH with no success.
Wondering what protocols you guys usually use for pombe transformation.
Any suggestions are welcome and highly appreciated.
Zhaomin
