In Article <3m70i0$giu at moe.cc.emory.edu>, anichol at larry.cc.emory.edu
(Ainsley Clare Bayliss) wrote:
>Hello all. I am using a his3/lac Z fusion protein and will need to make
>individual yeast colonies turn blue with Xgal. My colonies are turning
>blue, but they take forever to grow (on media that is pH 7.05) and even
>longer to turn blue, and so far they don't all turn blue. Does anyone
>have experience with plating yeast on Xgal?
I had this problem some years ago. In my case, I used hterologous promoters
to transcribe the LacZ gene. How blue cells turned depended on the
promoter strength. In some cases, I did use 72 hours as reaction time.
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