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Pichia vectors

Stu Cain s.a.cain at sheffield.ac.uk
Wed Sep 28 10:33:05 EST 1994


In article <CwIDvH.BuE at murdoch.acc.Virginia.EDU>, vlm4b at darwin.clas.Virginia.EDU (Vicky L. Miller) says:
>
>Dear fellow Pichia protein expressers,
>        This is not good news. It has taken me months to get my
>Dna into one of the secretion vectors. Tonight I plated from
>RDB plates onto the MM and MD plates. I'll let you know what
>happens. What concentration of antibiotic do you use in your
>media? 
>
>Vicky Miller
>Graduate Student whose life hangs in the balance


Dear Vicky
	I have found there is no need to add any antibiotics 
to any of your plates or media  as long as they are prepared
reasonably sterile.
Good Luck with your selection.

Stu Cain
Another graduate student whose at the mercy of Pichia pastoris



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