I agree that it is easier to transform with both plasmids at once, but I
didn't realize that you could transform two plasmids with 2 micron
replication origins into the same cell. I was always under the impression
that you had to have one on a centromeric plasmid. Please correct me if I
am mistaken, because it would make a big difference in an experiment I'm
currently setting up for this week.
Albany Medical College
BrianC4208 at aol.com