I'd be grateful if someone can give me advice on two points:
1) is it possible to recover live yeast cells after fusing them with
mammalian cells? I'm not experienced with yeast, though I have protocols
for yeast/ES cell fusions. I just wondered if it is possible to recover
yeast cells in some form after they have been fused with mammalian cells?
2) do the commonly used mammalian promoters/poly A signals (like PGK, CMV
etc) function in yeast. ie, is it possible to shuttle one selectable marker
cassette, like PGK-neo, between yeast and mammalian cells?
Thanks in advance,
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ