I have URA3 and LEU2 plasmids with 2 micron replication origins, and want
to look at growth of a strain maintaining both plasmids. Firstly, can I
do this and second, how do I transform the strain - put one plasmid in at
a time, or both together and select for both markers at once? I suppose
to use the second method I would need a pretty good transformation
frequency. Any suggestions appreciated.
University of Auckland, New Zealand, South Pacific.