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GAL4 nuclear localization signal

Colin Rasmussen ras-1 at bones.biochem.ualberta.ca
Fri Oct 21 21:08:17 EST 1994


In article <3894em$gms at mark.ucdavis.edu>, ez003450 at bullwinkle.ucdavis.edu
(George Theodoris) wrote:

> Regarding the 2-hybrid vectors which make fusions to the Gal4 activating 
> and DNA binding domains: Is there an easy way of removing the nuclear 
> localization signals from these vectors so that the hybrid proteins will 
> no longer be localized to the nucleus? Are there convenient restriction 
> enzyme sites that remove the nuclear localization signals from Gal4,
> without disrupting the reading frame?
>                                 -George


My question is why would you want to do this. If it's to purify a protein
there are lots of other ways. The two-hybrid idea depends on nuclear
localization.

Colin



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