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GAL4 nuclear localization signal

George Theodoris ez003450 at bullwinkle.ucdavis.edu
Fri Oct 21 14:23:02 EST 1994

Regarding the 2-hybrid vectors which make fusions to the Gal4 activating 
and DNA binding domains: Is there an easy way of removing the nuclear 
localization signals from these vectors so that the hybrid proteins will 
no longer be localized to the nucleus? Are there convenient restriction 
enzyme sites that remove the nuclear localization signals from Gal4,
without disrupting the reading frame?

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