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# Klett vs.OD

Tue Oct 18 10:15:57 EST 1994

```klett-Sumerson colorimeter scale is graduated in units proportional to OD.  The actual numerical values represent the OD devided by two, with the decimal point omitted.  For eg., a scale of 250 corresponds to OD of 0.500

The formula relating scale reading and OD is:

D = R X 2/1000

where D is density and R is the reading.

it is more accurate to relate the reading in terms of per cent transmittance but would require looking up a already computed chart  available in the manual accompanying the old klett meters.

or
1) get OD value from reading as above

2) subtract OD from 2.000
The result is the logarithm of the per cent transmittance.

OD=150 X 0.002 = 0.300

log% transmittance = 2.000- 0.300= 1.700
% transmittance = 50.2%

In article 5 at ouvaxa.cats.ohiou.edu, bhatt at OUVAXA.CATS.OHIOU.EDU writes:
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>                    Ohio University Electronic Communication
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>
>    Date:  18-Oct-1994 10:16am EST
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>      To:  Remote Addressee                     ( _MX%"yeast at net.bio.net" )
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>    From:  Tejal Bhatt                                Dept:  Biological Sciences
>           BHATT                                    Tel No:  (614) 593-4635
>
> Subject:  Klett vs.OD
>
>
> Hi.  Can someone tell me how Klett values are converted to OD values? Thanks.
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