klett-Sumerson colorimeter scale is graduated in units proportional to OD. The actual numerical values represent the OD devided by two, with the decimal point omitted. For eg., a scale of 250 corresponds to OD of 0.500
The formula relating scale reading and OD is:
D = R X 2/1000
where D is density and R is the reading.
it is more accurate to relate the reading in terms of per cent transmittance but would require looking up a already computed chart available in the manual accompanying the old klett meters.
1) get OD value from reading as above
2) subtract OD from 2.000
The result is the logarithm of the per cent transmittance.
eg. scale reading =150
OD=150 X 0.002 = 0.300
log% transmittance = 2.000- 0.300= 1.700
% transmittance = 50.2%
In article 5 at ouvaxa.cats.ohiou.edu, bhatt at OUVAXA.CATS.OHIOU.EDU writes:
>> Ohio University Electronic Communication
>>> Date: 18-Oct-1994 10:16am EST
>> To: Remote Addressee ( _MX%"yeast at net.bio.net" )
>> From: Tejal Bhatt Dept: Biological Sciences
> BHATT Tel No: (614) 593-4635
>> Subject: Klett vs.OD
>>> Hi. Can someone tell me how Klett values are converted to OD values? Thanks.
>>>>> Received: 18-Oct-1994 10:19am