Philip Heacock (pheacock at utmmg.med.uth.tmc.edu) wrote:
: Hi, I have some unusual questions:
: Will yeast genomic DNA clones express in mammalian cell lines without
: adding new promoters?
: Has anyone tried to screen a mutant eukaryotic cell line with yeast DNA to
: look for complementation?
: Are there any good E. coli to yeast to mammal shuttle vectors?
: Thanks for your help,
: -Philip Heacock
You might want to look at some of the work done with RAS genes. I
know that RAS from yeast can complement defects in mammalian RAS
and visa versa. I believe some of this work was done by either
Tatchell or Wigler.