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Library Amplification

Bob Barstead, Oklahoma Medical Research Foundation, 825 N.E. 13th Street, Oklahoma City, OK 73104 barsteadr at omrf.uokhsc.edu barsteadr at omrf
Thu Oct 13 04:22:50 EST 1994



In article <Pine.3.89.9410121241.A24295-0100000 at homer06.u.washington.edu>, moser at U.WASHINGTON.EDU (Michael Moser) writes:
> When we amplify our libraries we always do it on plates to reduce 
> selection against plasmids which may make E. coli unhappy.

I also amplify on plates to "reduce selection".  My question is on what is
this based?  Is there any evidence that there is less selection on plates?
Or is this just another urban legend.

> 
> Mike Moser                                             Tel: 206-543-5354
> Department of Biochemistry  SJ-70                      FAX: 206-685-1792
> University of Washington                          moser at u.washington.edu
> Seattle, WA  98195                          Make peace my beast is yeast
> 

Bob Barstead
barsteadr at omrf.uokhsc.edu



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