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hydroxyurea concentration?

Hieter Lab Hieter at jhuigf.med.jhu.edu
Thu Oct 6 18:39:16 EST 1994

In article <n1430840471.67029 at QuickMail.Yale.edu>,
David_Gonda at QUICKMAIL.CIS.YALE.EDU ("David Gonda") wrote:

>                       Subject:                              Time:  2:39 PM
>   OFFICE MEMO         hydroxyurea concentration?            Date:  10/4/94
> What concentration of hydroxyurea should be used to get a good S-phase arrest
> of S. cerevisiae in solid media and in liquid media?
> thanks in advance-
> David Gonda
> david_gonda at qm.yale.edu

You should use 0.1M HU for liquid cultures and 0.1 - 0.3 for solid medium.

J. Kroll and S. Tugendreich

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