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YAC transformations

WALMSLEY WALMSLEY at mh1.mcc.ac.uk
Thu Oct 6 05:58:37 EST 1994


Dear YAC-interested netters

We are having great difficulties in transforming YACs into yeast 
strains with any respectable level of efficiency.  At the moment we 
use the method of Burgers and Percival from Anal Biochem (1987), 
cited in "Biotechniques" (1990) in which melted slabs are added to 
sphaeroplasts.

Do you think you have the best method in the world? we'd like to see 
it!

Thanks a lot,

Richard Walmsley
tel 061 200 4174
fax 061 236 0409



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