I have been trying for some months now to get an interacting protein out
of the Clontech human brain library. I have read a few recent posts
regarding the generally poor quality of their libraries but I am writing
to ask whether anyone else has gotten anything out of the human brain
library with any type of bait (I need some encouragement!). I would like
to try another adult brain library (primate or rodent) but I don't have
any access-if anyone has one I would love to get a small amout to amplify.
BTW-my controls with baits and preys pertinent to our area of study have
all worked well. Also, I am using 2mg of bait and library to transform
and transformation efficiency is usually reasonable (about 10e5/ug). I
have run a southern on the Eco RI cut library with a relatively rare
pertinent gene and get multiple bands. But I just dont seem to get
anything out of the library-someone please tell me something!!!!
andybean at cmgm.stanford.edu