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myc-tagged proteins in S.c.

Irmgard Irminger Irminger at sc2a.unige.ch
Tue Nov 29 06:35:02 EST 1994


Did anyone use the myc-tag to localize specific proteins in Saccharomyces
cerevisiae by immunofluorescence with anti-myc antibodies and what was your
experience? 
Surprisingly we found that the anti-myc antibody localized to microtubules
in control cells that do not express a myc-tagged construct.  Did anybody
observe similar effects with certain antibody preparations? 



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