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mating yeast

Michael Lichten lichten%bchem.dnet at DXI.NIH.GOV
Sat Nov 26 20:27:13 EST 1994

Michael Kyba is having trouble mating yeast.

You don't have to play violins, but you ought to try treating them to a 
nice dinner.

It sounds like you are doing the matings directly on selective medium.  
Although this shortcut often works, it's still a shortcut.  You're asking 
the cells to grow enough in the absence of whatever they need to mate.  Try 
doing exactly what you're doing but on YEPD plates.  Let the shmush grow 
4hr-overnight, then replica-plate to selective plates.  

Michael lichten at helix.nih.gov

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