I try to perform a gene disruption with the construct pNKY51
(Alani, Cao, Kleckner: Genetics 116, 541-)
BUT............it doesn`t work.
So 1.) who has the plasmid pNKY51 and can send it to me (europe)?
2.) who used this technique with success and can give me some hints?
Thank you in advance.
Depatment og Genetics and General Biology
University of Salzbourg
email.: reinhard at dgen10.gen.sbg.ac.at