Dear Charlie,
I already have this vector in my hand, but my problem is that I
want to make exoIII-S1 from the 5' end of my promoter. If I could have a
plasmid with unique KpnI SalI and BamHI I would be very happy. The KpnI
or other 3' end site will protect by vector from digestion, and the SalI
and BamHI site will be used to clone my promoter. I just hope that a
vector with theses features exists.
Best regards,
JYM
On 22 Nov 1994, Hoffmacs wrote:
> Hi Jean-Yves et al.,
> I once altered a translational fusion vector (pSEYC102-centromeric plasmid
> with URA3 marker) to make a transcriptional fusion vector. Cloning sites in
> the polylinker are EcoRI-SmaI(XmaI)-BamHI-XhoI (although this site is
> repeated in the plasmid-for use if you want to pull out lacZ)-HindIII. The
> reference for this plasmid, pCHY22 is Gene 84:473-479. Let me know if you
> want it.
> Charlie Hoffman
> "I'd rather be fission (but I've done a few things in budding yeast too)."
> ________________________________________________________
> To: yeast at net.bio.net> From: Jean-Yves Masson on Mon, Nov 21, 1994 8:48 PM
> Subject: promoter-probe vector
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> To: yeast at net.bio.net> From: massonjy at rsvs.ulaval.ca (Jean-Yves Masson)
> Subject: promoter-probe vector
> Date: 21 Nov 1994 17:39:58 -0800
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>>> Hi everybody,
>> I am looking for a shuttle (between E. coli and yeast)
> promoter-probe plasmid. The vector should contain a good polylinker and
> also the lacZ gene as the reporter gene.
>> Thanks
>> Jean-Yves Masson
> Molecular Endocrinology Laboratory
> CHUL Research Center
> E-mail: MASSONJY at rsvs.ulaval.ca>>>>
