Ohio University Electronic Communication
Date: 22-Nov-1994 05:29pm EST
To: Remote Addressee ( _MX%"yeast at net.bio.net." )
From: Tejal Bhatt Dept: Biological Sciences
BHATT Tel No: (614) 593-4635
Subject: yeast shuttle vectors
Hi. I am looking for a yeast shuttle vector which will allow me to express and
secrete a protein. I have a cDNA flanked by a BAR1 promoter/secretory leader and
triose phosphate isomerase terminator, cloned into a vector with leu2-d and
triose phosphate isomerase (POT) selectable markers. The selection is
inefficient and the only method of transformation (spheroplasting) I have been
able to use with the host strain is- to put it kindly- a pain. I am not
experienced with yeast and only have a vague understanding of its genetics. I
only want to use yeast as a means to express my protein at high levels and for
easy purification and mutagenesis. Even though I do not know much about yeast, I
know there must be a yeast system which is "user friendly". If anyone could
refer me to a company which sells yeast expression vectors which: 1) contain
either a leu2-3, trp1, or ura3 selectable marker 2) contain a promoter/secretion
signal-termination sequence into which cDNA can be cloned- but not a fusion
protein 3) constituitively express 4) autonomously replicate and 5) are easily
shuttled from E. coli to yeast and back, I would really appreciate it ( I have
already looked through Stratagene and Clonetech). If anyone knows of such a
vector or has one that they would be willing to let me use, I would be most
grateful.
Tejal Bhatt
Dept. of Biol. Sci.
331 Irvine Hall
Ohio University
Athens, Ohio 45701
Received: 22-Nov-1994 06:12pm
