I would be grateful for help in getting hold of some of the strains and
plasmids used for shuttle mutagenesis of yeast DNA, as referenced in
Hoekstra et al, chapter 22 in Meth in Enz 194.
I realize there are quite a number of possible vectors and transposons
available, and before I bother individual labs for the strains, I want to
know if there is perhaps a stock center that now distributes these
materials?
many thanks, Eric Schott
p.s. the recent question from MASSONJY at rsvs.ulaval.ca about shuttle
vectors and promoter-probes might be well-answered by some of the Tn3
constructs listed in the Meth in Enz 194 chapter.