re need protocols for making agarose inserts from yeast

[goldman%bchem.dnet at DXI.NIH.GOV]

> need protocols for making agarose inserts from yeast

>Can anyone recommend a fast method for making agarose insert blocks from S.
>cerevisiae cultures ?  I recently saw a reference to a protocol  that skipped 
>the zymolyase or lyticase steps, but can't find the protocol.


The reference you want is 

Ibeas and Jimenez Nucleic Acids Research 1993 21:3902

And it works just fine.

ASHG