> need protocols for making agarose inserts from yeast
>Can anyone recommend a fast method for making agarose insert blocks from S.
>cerevisiae cultures ? I recently saw a reference to a protocol that skipped
>the zymolyase or lyticase steps, but can't find the protocol.
The reference you want is
Ibeas and Jimenez Nucleic Acids Research 1993 21:3902
And it works just fine.