(Put in whatever greeting suits you),
Has anyone gotten 5FOA to work in a defined minimal medium? We'd like to
regulate a promoter at the same time we subject our yeast to 5FOA. For this,
we cannot use yeast nitrogen base (because I assume it has thiamine in it-
can the pombe nmt users corroborate this?). I recall a previous effort by a
grad student in my lab to include 5FOA in a defined medium resulted in growth
of a control Ura+ strain, but we didn't pursue it at the time. Anyone else
look at this issue? Aside from asking for others' experiences, how about
addressing this theoretically? Why doesn't 5FOA kill a Ura+ strain growing
on a defined medium? Does this say something about uracil biosynthesis?
Thank you in advance for information and speculation.
"I'd rather be fission."