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5FOA

Hoffmacs Hoffmacs at HERMES.BC.EDU
Thu Nov 17 07:05:09 EST 1994


(Put in whatever greeting suits you),
Has anyone gotten 5FOA to work in a defined minimal medium?  We'd like to
regulate a promoter at the same time we subject our yeast to 5FOA.  For this,
we cannot use yeast nitrogen base (because I assume it has thiamine in it-
can the pombe nmt users corroborate this?).  I recall a previous effort by a
grad student in my lab to include 5FOA in a defined medium resulted in growth
of a control Ura+ strain, but we didn't pursue it at the time.  Anyone else
look at this issue?  Aside from asking for others' experiences, how about
addressing this theoretically?  Why doesn't 5FOA kill a Ura+ strain growing
on a defined medium?  Does this say something about uracil biosynthesis? 
Thank you in advance for information and speculation.
Charlie Hoffman
"I'd rather be fission."



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