>Fellow smaller (not lower) eukaryote enthusiasts,
>We did a primer extension that suggested transcription of a gene initiated
>one nucleotide upstream from an ATG in the sequence. Would such a
>transcript be likely successfully to have a ribosome initiate translation on
>the second through fourth nucleotides? Is anyone aware of such a short 5'
>untranslated region in any other gene (yeast or otherwise). Thanks for any
>input.
>Charlie Hoffman
>"I'd rather be fission."
My good friend Emmanuel Maicas has you beat by one! He saw initiation from
a leader of a TCM1 derivative with a mRNA starting at the A of the
initiation codon. See E. Maicas, Shago, M. and Friesen, J.D. (1990)
Translation of the Saccharomyces cerevisiae tcm1 gene in the absence of a
5'-untranslated leader.
Nucleic Acids Research 18: 5823-5828.
David Jansma
jansma at sickkids.on.ca
