These are interesting observations ... do you actually see a CAP
structure at the ATG? Has the protein been sequenced directly to show
that this ATG was being used?
| B.F. Francis Ouellette
||francis at ncbi.nlm.nih.gov
> >Fellow smaller (not lower) eukaryote enthusiasts,
> >We did a primer extension that suggested transcription of a gene initiated
> >one nucleotide upstream from an ATG in the sequence. Would such a
> >transcript be likely successfully to have a ribosome initiate translation on
> >the second through fourth nucleotides? Is anyone aware of such a short 5'
> >untranslated region in any other gene (yeast or otherwise). Thanks for any
> >Charlie Hoffman
> >"I'd rather be fission."
>> My good friend Emmanuel Maicas has you beat by one! He saw initiation from
> a leader of a TCM1 derivative with a mRNA starting at the A of the
> initiation codon. See E. Maicas, Shago, M. and Friesen, J.D. (1990)
> Translation of the Saccharomyces cerevisiae tcm1 gene in the absence of a
> 5'-untranslated leader.
> Nucleic Acids Research 18: 5823-5828.
> David Jansma
>jansma at sickkids.on.ca