An alternative method involves the enrichment for nongrowing cells due to
their resistance to heat killing. This comes from a paper from the Pringle
lab (MGG 171:111-114).
Charlie Hoffman
________________________________________________________
To: yeast at net.bio.net
From: "Preston Garrison" on Thu, Nov 3, 1994 11:47 PM
Subject: Re: Drug to kill actively growing yeast cells
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To: yeast at net.bio.net
From: garrisonp at THORIN.UTHSCSA.EDU ("Preston Garrison")
Subject: Re: Drug to kill actively growing yeast cells
Date: 3 Nov 1994 18:15:34 -0800
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>[ Article crossposted from bionet.molbio.methds-reagnts ]
>[ Author was Tony Kwan (kwan at medcor.mcgill.ca) ]
>[ Posted on Wed, 02 Nov 1994 22:46:46 -0500 ]
>>Hello netters,
>>I'm looking for a drug that would be able to kill yeast cells which are
>actively dividing and would leave those which aren't alone. Something
>analogous to penicillin in e.coli, for example. Any help would be
>appreciated.
>>--
>Tony Kwan
>Department of Biochemistry, McGill University
>kwan at medcor.mcgill.ca>>--
>| B.F. Francis Ouellette
>|>|francis at ncbi.nlm.nih.gov>
Nystatin has been used for that. See R. Snow, Nature 211: 206 1966.
There have been subsequent uses of the method, but I don't have references
handy. There are a couple of other methods described in McCusker and Haber,
Genetics 119: 303 1988, using ethidium bromide or fluorouracil. EthBr
apparently causes loss of mito DNA much more efficiently in growing cells
than in arrested ones. So the rho+ survivors are enriched in mutants.
Hope this helps,
Preston Garrison garrisonp at uthscsa.edu
Biochem. Dept. voice: 210-567-3702
Univ Texas Health Sci Ctr fax: 210-567-6595
San Antonio, Tx 78284-7760
USA
