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Olson lambda clones

Anne Rosenwald arosen at HELIX.NIH.GOV
Fri Nov 4 14:18:59 EST 1994


Hi Buddies!

I have some questions about the Olson lambda clones with the yeast
genome.  The clone I've been working on for several months (WashU 4190) is
supposed to be from chromosome XIII, but I just sequenced the fragment
with the piece I'm interested in and it is nearly identical (>97%) to a
gene we already know about on chromosome IV.  So here are my questions.

	1.  how often do these guys rearrange? is it only intramolecular? or
could fragments from other chromosomes get in here?  I should mention that
I got this clone from ATCC, and it clearly contains several different
clones, only about 20% of which have the fragment which lights up with my
probe.

	2. could this be at all biologically interesting?  pseudogene? an
exact (or nearly so) copy at another locus?

I have a few more experiments to do (like test a chromosome blot to see
if I can detect anything on chromosome XIII), but would appreciate feedback
from anyone who has worked with these clones and may have encountered
similar problems.

Thanks!!

Anne

Anne Rosenwald
arosen at helix.nih.gov




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