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PCR KO strategy

Steve Kron kron at WI.MIT.EDU
Fri Nov 4 18:25:14 EST 1994

1)  Yeah, even 150 basepairs on either end is ok (e.g. the PvuII sites
adjacent to the polylinker in blue white vectors).

2)  Yeah, we would be very interested as well if there is some concensus on
how well the PCR trick works in general (under conditions where you don't
know the KO phenotype ahead of time).

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