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PCR in yeast?

Hieter Lab hieter at jhuigf.bitnet
Thu Nov 3 13:16:30 EST 1994

In article <396fhm$mqn at mark.ucdavis.edu>, ez005528 at chip.ucdavis.edu (Kevin
Morano) wrote:

> Danny Altschuler (dla at galactose.mc.duke.edu) wrote:
> : Does anyone know any easy protocol to PCR from single yeast colonies?
> Danny,
> Check the molbio.methods-reagents archives for more detailed 
> correspondence, but the simple way is to grow a fresh plate of your yeast 
> and pick a small colony with your toothpick. Swizzle (scientific term) in 
> your PCR mix and go. I use hot start, which greatly improves specificity.

Danny, I think the above is basically the method of choice. I've had
results doing colony PCR, but one thing to avoid is touching the plate 
surface as you toothpick your colony, as agar is inhibitory to PCR.
John Puziss

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