[ Article crossposted from bionet.mycology ]
[ Author was John Bissett (BISSETTJ at NCCCOT2.AGR.CA) ]
[ Posted on 31 Mar 1994 13:58:11 -0800 ]
An industry client has an urgent need of a permeant vital
stain for Saccharomyces cerevisiae and other yeasts. Their
automated imaging system cannot reliably distinguish yeasts
on grid membrane filters from dust and other
autofluorescing contaminants. The stain should not be
toxic to yeasts. A fluorescent brightener should have an
emission range other than 415-505 nm, the range of
autofluorescence in this application.
Has anyone knowledge of vital stains/optical brighteners
for this application - or another solution?
Please address replies to: BISSETTJ at NCCCOT2.AGR.CA
| B.F. Francis Ouellette
||francis at ncbi.nlm.nih.gov