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Permeant vital stains for yeasts

Francis Ouellette francis at borduas.nlm.nih.gov
Thu Mar 31 18:23:42 EST 1994

[ Article crossposted from bionet.mycology ]
[ Author was John Bissett (BISSETTJ at NCCCOT2.AGR.CA) ]
[ Posted on 31 Mar 1994 13:58:11 -0800 ]

   An industry client has an urgent need of a permeant vital
   stain for Saccharomyces cerevisiae and other yeasts.  Their
   automated imaging system cannot reliably distinguish yeasts
   on grid membrane filters from dust and other
   autofluorescing contaminants.  The stain should not be
   toxic to yeasts.  A fluorescent brightener should have an
   emission range other than 415-505 nm, the range of
   autofluorescence in this application.
   Has anyone knowledge of vital stains/optical brighteners
   for this application - or another solution?
   Please address replies to:   BISSETTJ at NCCCOT2.AGR.CA
   John Bissett

| B.F. Francis Ouellette  
| francis at ncbi.nlm.nih.gov   

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