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GST

Michael Knop/Mlq knop at PO.UNI-STUTTGART.DE
Tue Mar 22 12:50:16 EST 1994


Hi


concerning your re-solubilized GST-fusion protein: are you aware of the fact 
that you might have denatured your GST reporterprotein with your extraction 
buffer. Sarkosyl is a strong detergent which may enfold the GST-protein.
To circumvent your problem try to grow up the E.coli cells at 30C which may 
prevent the formation of inclusion bodies, but do induce them for longer 
periodes, because metabolic activity is reduced at lower temperature. Also 
helpfull may be to fuse only parts of your favorite protein to the GST 
preferably those whith high antigenity and low hydrophobicity. A native GST-
protein exhibits as well an assayable activity, see pharmacia instruction.

good luck :-) wohi,mikn,stuttgart




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