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yeastplasmid rescue

Margaret M Lanterman ckrudy at bimcore.emory.edu
Sun Mar 13 16:33:17 EST 1994

I am trying to recover a cen plasid from yeast and transform E. Coli.  I have tried a boiling prep protocol that didn't work for me.  Does anyone have a protocol that always works?  Does the strain of Ecoli matter- I used HB101.
I really need this to work ASAP help!!!!
Margaret M Lanterman

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