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site specific cleavage of mRNA

Mauro Modesti cnglab at cmb.biosc.wayne.edu
Fri Mar 11 17:03:26 EST 1994


Hi, 

I want to use RNase H to do an site specific oligo-directed cleavage of a
given mRNA molecule.   The mRNA of interest is present in low abundance
in a polyA+ fraction of total yeast RNA.

Has anyone tried this approach?   Please send comments...

Thanks
Mauro
cnglab at cmb.biosci.wayne.edu



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