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oleic acid medium

Tomas Drgon DRGON at fns.uniba.sk
Wed Mar 2 15:05:50 EST 1994

Hi again.
Please do not read my previous message :)

I have found the original protocol:

20 g of oleic acid + 20 g of Tween 80 dissolve in 100 ml sterile
dest. water to obtain homogenous milky emulsion.
1.4 g of NaOH dissolve in 40 ml water and add slowly to the emulsion
while virgously stirring. Bring the volume to 200 ml. Boil once for
several minutes. Boil again after 24 hours to ensure sterility. The
pH should be round 7. Adjust if exceeds. Store in dark glass bottle
at RT.
The resulting emulsion contains 10% oleic acid.

Good luck again

Tomas Drgon

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