>> I'm not sure that I would add the fatty acid prior to autoclaving. We tend
> to autoclave the media first, let it cool, add the detergent, then deliver
> the proper amount of fatty acid in ethanol. This seems to work fairly well.
> I am curious as to why you use 0.5% fatty acid. Most of the receipes I have
> seen call for about 0.1% fatty acid (approximately 4 mM).
>> Let me know if I can be of further assistance.
>> Dubear Kroening
>dubear at molbio.cbs.umn.edu
We do it as follows:
-prepare 10% solution (emulsion) of oleic acid in sterile water with
-titrate to pH about 7 with NaOH
-boil on water bath for 15 minutes (semisterilisation)
-add to autoclaved media to 0.1% (depends what you do)
We use this for induction of peroxisomal enzymes in S.c.
Works well, 10% stock stays in solution ,no contamination.
Shelf life: several months at RT.
P.S.: For some refs try the name Rytka in MedLine.