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pichia pastoris growth

Ashwini Kamath-Loeb askamath at post.its.mcw.edu
Mon Jun 13 20:57:35 EST 1994

We are growing the P. pastoris yeast for expression studies and have noticed
some small round organisms.  We are concerned about possible contamination.
How can one differenciate between budding yeast and bacteria?  Can you grow
P.pastoris and express proteins in the presence of antibiotics?  How do
yeast appear following gram stain?

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